Figure 2.

af-MSCs enhance survival and plasticity of HAECs after hypoxia/reoxygenation. (A): Number of living HAECs after hypoxia and coculture with af-MSCs or HAECs during reoxygenation assessed by trypan blue staining. (B): Tubular structure sprouting of HAECs in HCM or ACM by counting number of branch points and number of tubes per well and representative pictures. Scale bars = 400 μm. Abbreviations: ACM, af-MSC-conditioned medium; af-MSC, amniotic fluid-derived mesenchymal stem cell; ECM, EGM-2MV-conditioned medium; EGM-2MV, EGM-2 microvascular medium; HAEC, human aortic endothelial cell; HCM, HAEC-conditioned medium.