Figure 1.

Erythroid differentiation of human pluripotent stem cells. (A): Schematic diagram of the protocol used for in vitro differentiation via sac formation used with hESCs and hiPSCs. hESCs and hiPSCs were differentiated into CD34⁺CD43⁺ hematopoietic progenitor cells within the sac structure in the presence of VEGF. (B): Schematic diagram of the differentiation protocol used for human CB CD34⁺ cell-derived erythroid cell differentiation. (C): Representative flow cytometric analysis of cell surface markers (CD235a, CD43, and CD71) in hESC H1-derived and CB CD34⁺ cell-derived erythroid cells. Days in culture are indicated above the plots. Abbreviations: CB, cord blood; EPO, erythropoietin; hESCs, human embryonic stem cells; hiPSCs, human induced pluripotent stem cells; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor.