Figure 5.

Overexpression of BCL11A-L in hESC and hiPSC-derived erythroid cells. (A): Schematic diagram of the DOX-inducible system for expression of human BCL11A-L. (B): Photomicrographs of GFP⁺ hESC H1 (hESC H1-BCL11A-L-GFP) and hiPSC 8 (hiPSC 8-BCL11A-L-GFP) cells on mouse embryonic feeder cells: left, phase contrast; middle, GFP; right, merged image. Scale bars = 100 μm. (C): Both cell lines were similarly GFP⁺ and SSEA-4⁺. Abbreviations: DOX, doxycycline; GFP, green fluorescent protein; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; SSEA-4, stage-specific embryonic antigen-4.