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. 2014 May 14;136(25):9001–9013. doi: 10.1021/ja5030194

Figure 3.

Figure 3

X-band cw EPR spectra (black) and simulations (red) of NrdD glycyl and thiosulfuranyl radicals. See the text and Table 1 for the simulation parameters. All experimental spectra were scaled to the simulation. The narrow signal of the G• was scaled by a factor of one-tenth for comparison with the broader signals of the new radical. The spectral artifact at 337.5 mT (ge) is due to an impurity in the cryostat. (i) The G• in activated NrdD. (ii–v) The thiosulfuranyl radical generated by reaction of CTP and ATP in the absence of formate with (ii) NrdD in H2O, (iii) [β-2H]-cys-NrdD in H2O, (iv) NrdD in D2O, and (v) [β-2H]-cys-NrdD in D2O.