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(A) Proposed mechanism for proton exchange on C175, which is prevented in NrdD-(S386E). (B) X-band cw EPR spectra (black) and simulations (red) of (i) NrdD-(S386E) in D2O and (ii) [β-2H]-cys-NrdD-(S386E) in H2O. See the text and Table 1 for the simulation parameters. The spectral artifact at 337.5 mT (∼ge) is due to an impurity in the cryostat.
