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. 2014 Jun 23;6:ecurrents.tol.cdf8b74881f87e3b01d56b43791626d2. [Version 1] doi: 10.1371/currents.tol.cdf8b74881f87e3b01d56b43791626d2

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Sliding window analyses of nucleotide diversity (π) along the “unique” alignment (see Methods). — The horizontal line indicates the average π for the fragment, 0.206. Analyses with window lengths of 10 bp and 100 bp were run with a 10 bp step size; both analyses showed lower variability on the 5’ end of the amplified fragment.