Table 1.
qPCR and microarray gene expression analyses of mammary tissue collected from negative (NEB) vs. positive energy balance (PEB) cows 20 h after an intramammary mastitis challenge with Streptococcus uberis
| Fold NEB vs. PEB |
|||
|---|---|---|---|
| Gene | Gene Name | Microarray1 | qPCR |
| ACADS‡ | acyl-coenzyme A dehydrogenase, C-2 to C-3 short chain | 1.66§ | −1.48§ |
| ACP2 | acid phosphatase 2, lysosomal | −1.49§ | −1.16§ |
| ADFP | adipose differentiation-related protein | −1.04 | −1.31§ |
| AKT1 | v-akt murine thymoma viral oncogene homolog 1 | −1.21§ | −1.70§ |
| ANXA1 | annexin A1 | 1.52§ | 1.14 |
| BAX | BCL2-associated X protein | −1.61 | 1.12 |
| BNBD5† | neutrophil β-defensin 5 | NA | 1.01 |
| C1QC | complement component 1, q subcomponent, C chain | 1.21 | −1.26 |
| C3 | complement component 3 | 1.22 | −1.42§ |
| CASP8† | caspase 8, apoptosis-related cysteine peptidase | NA | −1.04 |
| CCR1 | chemokine (C-C motif) receptor 1 | 1.31 | 1.58 |
| CD14 | CD14 molecule | −1.14 | −1.27 |
| COX1† | cyclooxygenase-1 | NA | −1.54§ |
| CSN2 | casein β | −1.12 | −1.50§ |
| FOS | v-fos FBJ murine osteosarcoma viral oncogene homolog | 1.32 | 2.31§ |
| HLA-A | major histocompatibility complex, class I, A | 1.83 | 9.39§ |
| HLA-DRA | major histocompatibility complex, class II, DR α | −1.05 | −1.30§ |
| HMOX1 | heme oxygenase (decycling) 1 | 1.56§ | 1.83* |
| IL1B | interleukin 1, β | −1.12 | −1.18 |
| IL1R2 | interleukin 1 receptor, type II | −1.05 | 1.36 |
| IL1RN | interleukin 1 receptor antagonist | 1.2 | −1.17 |
| IL6 | interleukin 6 | −1.29 | 1.03 |
| IL8 | interleukin 8 | 1.08 | 1.08 |
| IL10 | interleukin 10 | −1.34 | 1.68 |
| IL10RB | interleukin 10 receptor, β | −1.04 | 1.38 |
| IL15 | interleukin 15 | −1.06 | −1.05 |
| IL18 | interleukin 18 | 1.05 | 1.28 |
| INSIG1† | Insulin-induced gene 1, transcript variant 2 | NA | −1.67§ |
| INSR | insulin receptor | 1.24§ | 1.08 |
| IRAK1† | interleukin-1 receptor-associated kinase 1 | NA | −1.32§ |
| JAK1‡ | Janus kinase 1 (a protein tyrosine kinase) | 1.12 | −1.70§ |
| LTF | lactotransferrin | −1.01 | −1.30* |
| LYZ | lysozyme (renal amyloidosis) | 1.25 | −1.05 |
| MAPK9 | mitogen-activated protein kinase 9 | −1.04 | −1.28§ |
| NFKB1A | nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, α | 1.38 | −1.22 |
| NR3C1 | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) | −1.08 | −1.2 |
| SAA3 | serum amyloid A3 | −1.05 | 1.64 |
| SDHD | succinate dehydrogenase complex, subunit D, integral membrane protein | −1.55§ | −1.32§ |
| SELL | selectin L (lymphocyte adhesion molecule 1) | 1.02 | 1.56 |
| SELP | selectin P (granule membrane protein 140 kDa, antigen CD62) | −1.2 | −1.32 |
| SOCS2‡ | suppressor of cytokine signaling 2 | −1.45§ | 1.2 |
| SOD1 | superoxide dismutase 1, soluble [amyotrophic lateral sclerosis 1 (adult)] | −1.19 | −1.2 |
| SOD2 | superoxide dismutase 2, mitochondrial | −1.11 | 1.42 |
| SPARC‡ | secreted protein, acidic, cysteine-rich (osteonectin) | 1.25* | −1.90§ |
| SREBF1 | sterol regulatory element binding transcription factor 1 | −1.51§ | −1.3 |
| STAT3 | signal transducer and activator of transcription 3 (acute-phase response factor) | 1.04 | −1.12 |
| TLR2 | Toll-like receptor 2 | 1.07 | −1.28 |
| TLR4 | Toll-like receptor 4 | −1.07 | 1.09 |
| TNF | tumor necrosis factor (TNF superfamily, member 2) | −1.17 | 1.16 |
| TRAF6† | TNF receptor-associated factor 6 | NA | −1.29§ |
1
Differentially expressed genes on microarray were based on the adjusted P value false discovery rate (FDR) ≤0.06.
*
P ≤ 0.10;
§
P < 0.05.
†
Gene not present on microarray platform.
‡
Quantitative RT-PCR (qPCR) did not correspond to results obtained from differentially expressed genes by microarray. NEB, negative energy balance; PEB, positive energy balance; NA, not available.