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Western blot analysis of PKCα levels in RSM [in particulate or membrane (P) and cytosolic (C) fractions] in the basal state and following pretreatment with calphostin C, Gö-6850, and Y-27632 (all 10−5 M). Note significant (*P < 0.05) translocation of PKCα from membrane to cytosol after treatment with calphostin C and Gö-6850, but not Y-27632.
