Fig. 6.

Effect of Gly on CP-induced Evan's blue dye extravasation. Rats were subjected to the treatment regimen shown in Fig. 3. At the end of the experiment, rats were injected (intravenously) with 25 mg/kg Evans blue. One hour later, rats were euthanized and their bladders were harvested A: photograph of a representative bladder from each of the indicated treatment groups. B: Evans blue extracted from the bladders treated in the indicated ways. Bladders were weighed and incubated in formamide at 56°C overnight. Absorbance at 620 nm was analyzed, and measurements (in ng) of Evan's blue were determined from a standard curve. Bars are means ± SE; n = 4 for vehicle, 4 for CP, 4 for Gly, and 7 for Gly + CP treatment. *Significant (P < 0.05) differences from control as assessed by ANOVA modeling; †significant difference from vehicle + CP treatment.