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. 2009 Sep;107(3):853–858. doi: 10.1152/japplphysiol.00404.2009

Table 1.

Primer sequences used for qRT-PCR

PCR Primer Sequence 5′→3′ Melt Curve, °C Average CT
IL-6 F: CTA TGA ACT CCT TCT CCA CAA CGC CTT 82 21.3
R: GGG GCG GCT ACA TCT TTG GAA TCT T
IL-8 F: GCT CTG TGT GAA GGT GCA GTT TTG CCA A 82 20.5
R: GGC GCA GTG TGG TCC ACT CTC AAT
IL-15 F: CCG TGG CTT TGA GTA ATG AGA ATT TCG AA 79 20.9
R: CCT GCA CTG AAA CAG CCC AAA ATG AA
TNF-α F: CCC AGG CAG TCA GAT CAT CTT CTC GAA 85 19.6
R: CTG GTT ATC TCT CAG CTC CAC GCC ATT
GAPDH F: AGC CAC ATC GCT CAG ACA 79 18.3
R: GCC CAA TAC GAC CAA ATC C

qRT-PCR, quantitative real-time PCR; F, forward; R, reverse; CT, cycle threshold.