Table 1.
PCR Primer Sequence 5′→3′ | Melt Curve, °C | Average CT | |
---|---|---|---|
IL-6 | F: CTA TGA ACT CCT TCT CCA CAA CGC CTT | 82 | 21.3 |
R: GGG GCG GCT ACA TCT TTG GAA TCT T | |||
IL-8 | F: GCT CTG TGT GAA GGT GCA GTT TTG CCA A | 82 | 20.5 |
R: GGC GCA GTG TGG TCC ACT CTC AAT | |||
IL-15 | F: CCG TGG CTT TGA GTA ATG AGA ATT TCG AA | 79 | 20.9 |
R: CCT GCA CTG AAA CAG CCC AAA ATG AA | |||
TNF-α | F: CCC AGG CAG TCA GAT CAT CTT CTC GAA | 85 | 19.6 |
R: CTG GTT ATC TCT CAG CTC CAC GCC ATT | |||
GAPDH | F: AGC CAC ATC GCT CAG ACA | 79 | 18.3 |
R: GCC CAA TAC GAC CAA ATC C |
qRT-PCR, quantitative real-time PCR; F, forward; R, reverse; CT, cycle threshold.