Figure 3.

Fluorescence micrograph of the typical neurite field extending from DRG seeded on double-layered PCL nanofiber scaffolds (with aligned fibers in the top layer and random fibers in the bottom layer) in the (A) absence and (B) presence of preseeded Schwann cells. The neurites were stained with Tuj1 marker (green). Inset: SEM image of the nanofiber scaffold with aligned and random nanofibers in the top and bottom layers, respectively. (C) Double staining of the Schwann cells in (B) with anti-S100 (red) and their nuclei with DAPI (blue). (D) Triple staining of the Schwann cells with anti-S100 (red), their nuclei with DAPI (blue), and the neurites with Tuj1 marker (green) for the sample shown in (B).