Fig. 1.

Effects of eukaryotic elongation factor (eEF) 2 kinase (eEF2K) inhibitor NH125 or A-484954 on TNF-α-induced expression of VCAM-1 (A, n = 5; C, n = 6–8) and endothelial-selectin (E-selectin; B, n = 6; D, n = 4) in cultured human umbilical vein endothelial cells (ECs) (HUVECs). After HUVECs were treated with 10 ng/ml TNF-α for 6 h in the absence or presence of NH125 (1 μmol/l, pretreatment for 30 min) or A-484954 (1–10 μmol/l, pretreatment for 30 min), expression of VCAM-1 and E-selectin was determined by Western blotting and shown as fold increase relative to control (cont; no-drug treatment). Equal protein loading was confirmed using total actin antibody. **P < 0.01 vs. cont; #P < 0.05 vs. TNF-α.