Fig. 10.

Effects of long-term NH125 treatment on expression of eEF2K (A), VCAM-1 (B), and E-selectin (C), and ROS production (D) in isolated mesenteric artery. After NH125 was administered to SHR (4 wk old) or age-matched WKY subcutaneously at a dose of 500 μg·kg⁻¹·day⁻¹ for 6 wk, superior mesenteric artery was harvested. Expression of eEF2K, VCAM-1, and E-selectin was determined by Western blotting and shown as fold increase relative to WKY (n = 6). Equal protein loading was confirmed using total-actin antibody. The frozen sections (5 μm) of the mesenteric arteries were made. They were loaded with H₂DCFDA (5 μmol/l) for 30 min, and the fluorescence images were obtained. Fluorescent intensity (n = 6) was shown as fold increase relative to WKY. **P < 0.01 vs. WKY; #P < 0.05, ##P < 0.01 vs. SHR.