Fig. 11.

Effects of long-term NH125 treatment on ACh-induced endothelium-dependent relaxation (A) and vascular hypertrophy (B) in mesenteric artery from SHR. A: after NH125 was administered to SHR (4 wk old) or age-matched WKY subcutaneously at a dose of 500 μg·kg⁻¹·day⁻¹ for 6 wk, superior mesenteric artery was harvested. Concentration-contraction relationships to ACh were shown (n = 4–6). ACh (0.1–300 nmol/l) was cumulatively added after the precontraction induced by submaximal doses of noradrenaline had reached a steady state. 100%, noradrenaline-induced precontraction. Results were expressed as means ± SE. B: after NH125 was administered to SHR (4 wk old) or age-matched WKY subcutaneously at a dose of 500 μg·kg⁻¹·day⁻¹ for 6 wk, superior mesenteric artery was harvested. The frozen sections (5 μm) were stained with hematoxylin and eosin. Thickness of arterial media (n = 6) was shown as fold increase relative to WKY. Representative photomicrographs were shown. Arterial media (M) and adventitia (A) lesions were shown. Scale bar, 50 μm. *P < 0.05, **P < 0.01 vs. WKY; #P < 0.05, ##P < 0.01 vs. SHR.