Fig. 2.

Effects of eEF2K inhibitor NH-125 (A) or A-484954 (B) on TNF-α-induced eEF2K phosphorylation in HUVECs. After HUVECs were treated with 10 ng/ml TNF-α for 5 min in the absence or presence of NH125 (0.1–1 μmol/l, pretreatment for 30 min) or A-484954 (1–10 μmol/l, pretreatment for 30 min), eEF2K phosphorylation (A, n = 4; B, n = 4) was determined by Western blotting and shown as fold increase relative to control (no drug treatment). Equal protein loading was confirmed using total eEF2K antibody. *P < 0.05, **P < 0.01 vs. cont; ##P < 0.01 vs. TNF-α.