Fig. 6.

Colocalization results of alternatively activated markers (CD206 and CD163), classically activated markers (CCR2 and CCR5), and growth mediators (MMP-9, MMP-2, VEGF, and OSM) with tumor-associated macrophages (TAMs) and tumor-associated neutrophils (TANs) (+ denotes positive colocalization, ++ denotes strongly positive colocalization, and − denotes no identifiable colocalization). TAMs colocalized to alternatively activated markers and did not show colocalization to either classical activation marker. TANs did not colocalize with either classical or alternative activation markers. Colocalization of growth mediators suggest polarization of expression, with TANs specifically colocalizing with MMP-9, and TAMs colocalizing with OSM and VEGF.