Table 2.

Sodium and volume load after saline injections and repletion

	Weight, g	Sodium Injected, mg	Sodium Consumed, mg	Sodium/Weight, mg/g	Saline Injected, ml	Saline Consumed, ml	Water Consumed, ml	Liquid/Weight, ml/g
Injection
0.15M NaCl	321.5 ± 9.22	6.89 ± 0	N/A	0.022 ± 0.001	2.0 ± 0	N/A	N/A	0.006 ± 0.000
2M NaCl	328.67 ± 5.34	91.96 ± 0	N/A	0.28 ± 0.004	2.0 ± 0	N/A	N/A	0.006 ± 0.000
Repletion
0.15M NaCl	175.5 ± 4.99	N/A	237.08 ± 26.69	1.35 ± 0.13	N/A	68.75 ± 7.74	N/A	0.39 ± 0.04
2M NaCl	359.5 ± 18.75	N/A	214.57 ± 19.39	0.6 ± 0.05	N/A	4.67 ± 0.42	20.17 ± 3.96	0.07 ± 0.01

Values are means ± SE. N/A, not available. Sodium manipulations were used to elevate c-Fos activation patterns in epithelial sodium channel (ENaC)-expressing neurons of the sensory circumventricular organs (CVOs) The rats that received hypertonic saline injections were given 13 times more sodium per body weight than the animals receiving isotonic saline injections. Furthermore, sodium-repleted rats had sodium/body weight levels that greatly exceeded the levels seen after the hypertonic saline injections. This increase was by a factor of 5. The 0.15M NaCl repletion rats consumed 2.25 more times sodium per gram of body weight than the 2M-repleted rats. Sodium-repleted rats ingested more volume per body weight than was injected into the rats treated with hypertonic saline. The rats used in the 2M NaCl repletion and 0.15M NaCl repletion experiments drank 11 and 64 times, respectively, more volume per body weight than was injected.