Fig. 2.

Promoter SNPs −1899T/G and −1785G/C modified nuclear protein binding affinity by electrophoretic mobility shift assays (EMSA). A: examining allele-specific effects of S1PR3 −1899T/G. Biotin-labeled oligonucleotide probes contains either −1899T, −1899G, or −1899del (deletion of −1899T/G), with or without competitor containing sequence for caudal type homeobox 1 (Cdx1) specific binding element. B: examining allele-specific effects of S1PR3 −1785G/C. One milligram of HL60 nuclear extract proteins and 5 pg/ml of biotin-labeled oligonucleotide probes contain either −1785G, −1785C, or −1785del (deletion of −1785G/C), with or without competitor containing sequence for early B-cell factor 1 (Ebf1) specific binding element.