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Inhibition of PP2A prevents acetaldehyde-induced tight junction disruption in mouse ileum ex vivo. Opened segments of mouse ileum were incubated with or without fostriecin for 20 min prior to exposure to 400 μM acetaldehyde for 1 h. Cryosections of these tissues were fixed and double-stained for occludin (green) and ZO-1 (red). Images were collected by using a laser scanning confocal microscope. This experiment was repeated twice with similar results.
