Table 1.
Antibodies used for different experiments
| Working Solution |
|||||
|---|---|---|---|---|---|
| Antibody* | Host Species | Vendor | Catalog No. | IB | IF |
| Arp3 | Mouse | Sigma-Aldrich | A5979 | 1:3,000 | 1:200 |
| Eps8 | Mouse | BD Biosciences | 610143 | 1:5,000 | 1:100 |
| FAK | Rabbit | Millipore | 06-543 | 1:1,000 | |
| Nectin-2 | Goat | Santa Cruz Biotechnology | sc-14802 | 1:75 | |
| Nectin-3 | Goat | Santa Cruz Biotechnology | sc-14806 | 1:300 | 1:75 |
| Palladin | Rabbit | Protein Tech Group | 10853-1-AP | 1:1,000 | 1:100 |
| Goat IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2350 | 1:3,000 | |
| Rabbit IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2370 | 1:3,000 | |
| Mouse IgG-HRP | Bovine | Santa Cruz Biotechnology | sc-2371 | 1:3,000 | |
| Goat IgG-Alexa Fluor 555 | Donkey | Invitrogen | A21432 | 1:200 | |
| Rabbit IgG-Alexa Fluor 555 | Goat | Invitrogen | A21429 | 1:250 | |
| Mouse IgG-Alexa Fluor 555 | Goat | Invitrogen | A21424 | 1:250 | |
IB, immunoblot; IF, immunofluorescence analysis; Arp3, actin-related protein 3 that induces branched actin polymerization; Eps8, epidermal growth factor receptor pathway substrate 8; FAK, focal adhesion kinase; HRP, horseradish peroxidase.
*
Antibodies used herein cross-reacted with the corresponding proteins in rats as indicated by the manufacturer.