Table 1. Quantification of archaeal and bacterial signatures via qPCR, FISH and SR-FTIR (values in brackes give standard deviation).
Method | Measurand | MSI-BF | SM-BF | SOPC |
QPCR (per ng DNA) | Archaeal 16S rRNA genes | 2.89E+06 (±5.63E+05)* | 2.09E+06 (±1.08E+06) | 2.03E+05 (±5.48E+04) |
Bacterial 16S rRNA genes | 7.48E+04 (±7.65E+03)* | 2.20E+04 (±2.68E+03) | 5.75E+05 (±1.09E+05) | |
DsrB genes | 5.12E+03 (±7.87E+02)* | 1.97E+03 (±9.82E+02) | 3.46E+02 (±1.25E+02) | |
Percent Archaea | 97.44%* | 98,96% | 26,09% | |
FISH | Percent archaeal cells | 92.96 (±2.16) | 86.41 (±7.02) | ND |
Percent SRB385 stained Bacteria | 85.4 (±4.7)* | 39.32 (±11.81) | ND | |
Percent DeltaMix stained Bacteria | 89.2 (±0.9)* | 63.87 (±14.70) | ND | |
SR-FTIR | Percent archaeal biomass | 97.0 (±6.0) | 97.1 (±4.4) | 38.7 (±13.8) |
*data from Probst et al., 2013.
ND: Not Determined.