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. 2014 Jun 27;9(6):e100373. doi: 10.1371/journal.pone.0100373

Figure 1. LRP/LR co-localises with PS1 of the γ-secretase complex.

Figure 1

(A) Co-localisation between PS1-GFP and LRP-dsRed is shown for HEK293 cells in the maximum intensity profile for PS1-GFP and LRP-dsRed. The 2D cytofluorogram confirms co-localisation as a diagonal is observed passing through quadrant 3. A diagonal is indicative of a high degree of co-localisation. Line profile displaying the fluorescence intensities of the 2 colour channels along a line of interest and reveals co-localisation occurring specifically within the cytoplasmic regions. Line profile reveals aligned spectra suggestive of high degree of co-localisation. (B) SR-SIM reveals that PS1-GFP and LRP-dsRed show a high degree of co-localisation in HEK293 cells. A diagonal passes through quadrant 3 of the 2D cytofluorogram suggesting that there is still a high degree of co-localisation between the 2 proteins at this resolution (80 nm). Scale bar: 5 µm. (C) SR-SIM reveals that co-localisation between LRP-dsRed and PS1-GFP is maintained in IMR-32 cells, although the 2D cytofluorogram is skewed by the differences in transfectability with the different plasmids. Scale bars: 5 µm.