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. 2014 Jun 27;9(6):e101001. doi: 10.1371/journal.pone.0101001

Figure 4. Cep192 knockdown causes a positive shift in acetylated tubulin density in U2OS cells.

Figure 4

A) Quantitative immunofluorescence of cells immunostained for alpha-tubulin showed no significant shift in total interphase MT polymer mass between control and Cep192 depleted cells. Vertical bars represent S.E.M. P value is 0.5951 N≥20 cells per experiment from 3 independent experiments. B) Immunofluorescence micrographs showing control and Cep192 siRNA-treated U2OS cells double labeled for acetylated tubulin and α-tubulin. C) Western blot control and Cep192 siRNA-treated U2OS cell lysates stained for acetylated tubulin. Densitometry measurements indicated that acetylated tubulin increases ∼64% following Cep192 knockdown. GAPDH is shown as a loading control. D) Levels of tyrosinated tubulin decrease 9% following Cep192 KD.