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. 2014 Jun 27;9(6):e101001. doi: 10.1371/journal.pone.0101001

Figure 8. MT nucleation following Ice Depolymerization in Cep192-2 Flp-In T-Rex U2OS cells.

Figure 8

A) Following 40 minutes of ice depolymerization, cells were incubated in 37°C media for 30 seconds to allow regrowth. Cells were fixed and stained with EB1 and Cep192. B) Following Cep192 depletion fewer MTs regrew(6.0±0.297 MTs/centrosome) than in controls (11.0±0.319 MTs/centrosome). Induction of Cep192-2 rescued the MT nucleating capacity (12.3±0.611 MTs/centrosome) of the centrosome to control levels. Depletion of Pericentrin lead to a significant increase in the number of MTs attached to the centrosome (16.4±1.54) relative to controls. Vertical bars represent S.E.M. P values are <0.001, P = 0.0691, and P = 0.0096, respectively. N≥14 cells per experiment from 3 independent experiments per condition.