Fig. 9.
Octopamine and forskolin increase levels of RhOps1-2 and RhGqα during daytime dark adaptation in vitro. LEs were dissected from animals at midday. Each LE was cut in half to yield four slices from each animal. One slice from each animal was fixed immediately in the light. The other three slices were incubated for 4 h at room temperature in the dark in OCM + 0.08% DMSO and one of the following: Dk, no further additions; OA, octopamine + IBMX; or FSK, forskolin + IBMX. RhOps1-2, RhOps5, RhOps Gqα and RhArr were quantified in each slice as described in the Materials and methods and the legend to Fig. 2. Data are expressed as mean (±s.e.m.) immunoreactive intensities per μm2 of rhabdom. Levels in each dark-adapted slice of an animal were normalized to those in the light-adapted slice of the same animal when the light adapted level is expressed as 1. Mean ± s.e.m. relative levels are presented for the number of animals shown in the first bar of each data set. Asterisks indicate significant differences among the dark-adapted treatments (one-way ANOVA followed by the Student–Newman–Keuls test; **P<0.01, *P<0.05).