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. 2012 Dec 1;125(23):5745–5757. doi: 10.1242/jcs.109769

Fig. 4.

Fig. 4.

Loss of Drp1 induces chromosomal instability and centrosome overamplification. (A,B) Loss of Drp1 induces chromosome abnormalities in mitosis. (A) Mitotic chromosomes were visualized in the control cells and Drp1-deficient cells stably expressing pAcGFP1-Mito by DAPI staining. Microtubules were visualized by staining cells with Alex Fluor 555-conjugated anti-β-tubulin antibody. The images show representative cells in metaphase and anaphase. Arrows indicate lagging chromosomes. The bars indicate 5 µm. (B) The percentage of mitotic control cells and Drp1-deficient cells with abnormal chromosomes was determined by counting at least 30 mitotic cells from three independent slides. These data represent the mean ± the standard deviation (s.d.). *** P<0.005. (C-E) Loss of Drp1 induces centrosome overamplification. (C) Centrosomes in control and Drp1-deficient cells stably expressing pAcGFP1-Mito were visualized by staining cells with anti-γ-tubulin antibody, followed by secondary Alex Fluor 594 goat anti-mouse antibody. The nuclei were visualized by DAPI staining. Arrows indicate the centrosomes. The bars indicate 10 µm. (D) Enlarged images of box a and box b in panel C of a single focal plane from Drp1 knockdown cells. (E) The percentage of control and Drp1-deficient cells with more than two centrosomes was determined by counting at least 100 cells from three independent slides. These data represent the mean ± the standard deviation (s.d.). ***P<0.005.