Table 1.
Effects of mutations in PCNA binding domains PIP1 and PIP2 in Polκ on TLS opposite the 5R,6S Tg lesion carried on the leading strand template of SV-40 based plasmid in human fibroblast (HF) cells
| siRNA | Vector expressing | # of Kan+ colonies | # of blue colonies among Kan+ | TLS (%) |
|---|---|---|---|---|
| NC | --- | 462 | 114 | 24.7 |
| Polκ | --- | 384 | 52 | 13.5 |
| Polκ | No Polκ | 276 | 42 | 15.2 |
| Polκ | WT Polκ (1-870) | 168 | 47 | 28.0 |
| Polκ | C-terminal Δ of Polκ (1-856) | 154 | 42 | 27.3 |
| Polκ | Polκ pip1 mutation (F532A, L533A) | 136 | 35 | 25.7 |
| Polκ | Polκ pip2 mutation (F868A, F869A) | 112 | 30 | 26.8 |
| Polκ | Polκ pip1 and pip2 mutations (F532A, L533A, F868A, F869A) | 206 | 25 | 12.1 |
| Polκ | Polκ pip1 mutation (F532A, L533A) with C-terminal Δ (1-856) | 148 | 23 | 15.5 |