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. 2012 Sep 15;139(18):3392–3401. doi: 10.1242/dev.079442

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Fig. 6. — Mouse Corl1 binding to Smad3 is enhanced by TGFβ/Activin signaling. (A) The interaction of FLAG-tagged mouse Corl1 and Ski with Myc-tagged mouse Smads was examined by immunoprecipitation followed by immunoblotting in 293T cells. Constitutively active ALK5 and ALK3 (CA-ALK5 and CA-ALK3) tagged with HA were used to activate TGFβ/Activin and BMP signal transduction, respectively. Top panel shows that mouse Corl1 strongly binds Smad3 when stimulated by activated receptor. The lower three panels indicate expression levels for each protein in the experiment. (B) Examination of the ability of CA-ALK5 (TGFβ/Activin receptor) to stimulate interaction of Smad3 with mouse Corl1 and Ski shows activated receptor induces an increase in Smad3 binding by both proteins and that Ski has greater affinity for Smad3 than mouse Corl1.