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. 2013 Apr 15;140(8):1720–1729. doi: 10.1242/dev.092304

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© 2013. Published by The Company of Biologists Ltd

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Fig. 4. — Dll4^+/- mice show reduced functional recovery after femoral artery occlusion. (A) Representative laser Doppler flow (LDF) images before and at various time points after femoral artery occlusion (FAO). (B) LDF perfusion shown as ratio of right to left hindlimb (R/L ratio) in wild type (n=20) and Dll4^+/- (n=6). There is reduced perfusion recovery in Dll4^+/-. (C) Ischemia score after FAO shows increased ischemia in Dll4^+/-. (D,E) Quantitative analyses of capillary density (D) and capillary-to-fiber ratio (E) on sections of the gastrocnemius muscle. (F,G) Assessment of vascular leakage using Evans blue dye in wild-type and Dll4^+/- adductor muscle (F) and gastrocnemius muscle (G) at 7 days after FAO. Pre, pre-ligation; post, immediately after ligation. L, left hindlimb; R, right hindlimb. (F,G) Day 3: wild type, n=6; Dll4^+/-, n=6. Day 7: wild type, n=8; Dll4^+/-, n=4. ^*P<0.05, ^**P<0.01, ^***P<0.001.