Fig. 2.

Loss of epithelial Gpr177 leads to limited alveolar development in the E18.5 lung. (A) Proximal-distal specification of epithelial progenitor cells is unaffected in the E14.5 mutant lung. Proximal and distal epithelial progenitor cells are labeled with Sox2 and Sox9, respectively. (B) Sox2 is maintained in the proximal airways of the normal and mutant lungs at E18.5. (C,D) Clara cells (Scgb1a1⁺) and ciliated cells (α-acetylated tubulin⁺) are present in the proximal airways of the normal and mutant lungs. (E-G) The numbers of type I cells (T1α⁺, arrowheads) and type II cells (SpC⁺ and ABCA3⁺, arrowheads) are reduced in the mutant lungs. The insets are the high magnification view of the boxed region. Scale bars: 50 μm.