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. 2011 Jun 15;138(12):2451–2456. doi: 10.1242/dev.058362

Fig. 2.

Fig. 2.

ChIP detects the in vivo association of GFP-LUG and GFP-SEU with miR172 promoter sequences. (A) ChIP assays with anti-GFP antibody and chromatin isolated from 35S:GFP-LUG; lug-16 (gray) and non-transgenic wild-type (white) A. thaliana inflorescences. Fold enrichment was quantified by qPCR using primers that flank putative AP2 binding sites. Error bars indiate s.d. of six replicates (two biological replicates with three technical repeats). (B) ChIP assays using anti-GFP antibody and chromatin from ProSEU:GFP-SEU in wild type (gray) and ap2-2 (white). Fold enrichment for the same primer sets as in A is shown. Error bars indiate s.d. of three technical replicates of one biological experiment.