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. 2012 Dec 15;125(24):6117–6126. doi: 10.1242/jcs.113381

Fig. 5.

Fig. 5.

miR-34c-5p mediates hypoxia-induced downregulation of mRNA and protein levels of sGCβ1 in mouse pulmonary smooth muscle cells. (A) Cells were exposed to 1% O2 under control conditions or treated with miR-34c-specific sponge for 24 hours. mRNA expression of sGCβ1 was determined by qRT-PCR. The levels of mRNAs were relative to the control GAPDH. Data are expressed as means ± s.e.m. (n = 4; **P<0.01 versus normoxia, #P<0.05 versus hypoxia). (B) Western blot analysis of sGCβ1 subunit expression in mouse pulmonary smooth muscle cells exposed to 1% O2 under control conditions or treated with miR-34c-specific sponge for 24 hours. Expression levels are normalized to β-actin and expressed as means ± s.e.m. (n = 4; **P<0.01 versus normoxia, #P<0.05 versus hypoxia).