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Hif-1α has no effect on the expression of miR-34c-5p during hypoxia in pulmonary smooth muscle cells. (A) Western blot to confirm knockdown of Hif-1α protein induced by lentivirus shRNA for Hif-1α during hypoxia. (B) Analysis of the expression of miR-34c-5p in pulmonary smooth muscle cells treated with 1% O2 or shRNA for Hif-1α. The levels of miRNAs were relative to the control 5 s rRNA. Data are expressed as means ± s.e.m. (n = 3; #P<0.01 versus normoxia). NS, non-significant.
