Fig. 3.

A mutation in the TLC1 template eliminates sequence pattern differences. (A) Top panel shows alignment of a telomeric primer ending in 5′-TGGGTGTGG-3′ with the TLC1 template to generate a single TG dinucleotide prior to the following core motif. The black horizontal line indicates the position of complementarity. Complementarity might continue through the following two template positions (gray bars) if synthesis in the previous round extends to the end of the TLC1 template (see text for detail). Newly synthesized nucleotides are highlighted in gray. Bottom panel shows percentage of core motifs associated with a GG dinucleotide (5′-TGGGTGTGG-3′) that is followed by the indicated number of TG dinucleotides before the subsequent core motif. Black bars, EST2 telomeres; gray bars, est2-LT^E76K telomeres. (B) Same as (A) except the template used is from the tlc1-KF10 allele. (C) Southern blot of telomere length in GA426 tlc1::KAN strains expressing EST2 (lanes 1–4) or est2-LT^E76K (lanes 5–8) from the endogenous locus and complemented with plasmids expressing TLC1 (lanes 1,2,5,6) or tlc1-KF10 (3,4,7,8). Marker sizes are in kilobases (kb).