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. 2011 Dec 15;138(24):5357–5368. doi: 10.1242/dev.065565

Fig. 1.

Fig. 1.

DMME recapitulated the early Flk1 expression pattern at 7.5 dpc. Whole-mount (A,B,G) and a transverse section (E) of X-gal stained Flk1-lacZ knock-in embryos. Whole-mount (C,D,H) and a transverse section (F) of X-gal stained Flk1-DMME-lacZ Tg embryos obtained from a stable transgenic line (line#711). The transverse section at 7.5 dpc showed lacZ expression in the mesoderm, but not in the primitive streak, similar to endogenous Flk1 expression (E,F: corresponds to the notation ‘E’ and ‘F’ in A,B). lacZ expression directed by Flk1-DMME gradually becomes restricted to caudal lateral mesoderm, but it was not detectable in blood vessels at 8.5 dpc (G,H). (I) DMME recapitulated early mesodermal expression of Flk1. A Flk1-DMME-GFP transgenic embryo at E7.5 (OB stage) was sectioned at different levels and subjected to immunohistochemistry with anti-Flk1 and anti-GFP antibodies. Scale bars: 100 μm in A,B,C,D,G,H; 50 μm in E,F. BV, blood vessels; LM, lateral mesoderm; PS, primitive streak.