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. 2014 Jun 25;14:200. doi: 10.1186/1472-6882-14-200

Figure 5.

Figure 5

ROS/GSK3β-dependent ATF3 expression and ROS-dependent cyclin D1 proteasomal degradation by MRBE. (A, B) SW480 cells were pre-treated with PD98059 (20 μM), SB203580 (20 μM), SB216763 (20 μM) or NAC (20 mM) for 2 h and then co-treated with MRBE (25 μg/ml) for 1 h. Cell lysates were subjected to SDS–PAGE and the Western blot was performed using antibodies against ATF3 and actin. (C, D, E) SW480 cells were pre-treated with MG132 (5 and 10 μM), PD98059 (20 μM), SB203580 (20 μM), SB216763 (20 μM) or NAC (20 mM) for 2 h and then co-treated with MRBE (25 μg/ml) for 1 h. Cell lysates were subjected to SDS–PAGE and the Western blot was performed using antibodies against cyclin D1 and actin. Actin was used as an internal control and DMSO was used as a vehicle.