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. 2014 Jun 19;7:277. doi: 10.1186/1756-3305-7-277

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Copyright © 2014 Lawton et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phylogenetic tree based on the ITS1 sequences representing different populations of Trichobilharzia franki from European countries and the snail species that they infect. The samples sequenced from Hampshire, UK cluster within a France-specific clade, the only country specific clade to emerge during analysis. The tree was constructed using the ML method in MEGA5 using the K2 + G substitution model. The scale shows the number of nucleotide substitutions per site between DNA sequences and nodal supports were generated using 1000 bootstrap replicates. The nodal support is given in NJ, ML and MP bootstraps respectively and only values >50 for at least two of the analyses are shown.