Highlights of the single cell study. Single cell behavior differs due to heterogeneity, which is a resultant of one of the genetic, biochemical/metabolic, physiological or behavioral heterogeneity. Additional benefits such as observation of discrete and dynamic events of cell during cell’s life-time, cellular pathways study without interference of neighbor cells, comparison or relating microscopic to macroscopic (single cell to a large population of cell) and study or rare and transient cell states can only be achieved by single cell analysis.