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An in vitro protocol to examine the generation of ECMs that bind leukocytes. Stromal cells such as ASMCs, lung fibroblasts, or synoviocytes are transduced with matrix genes or exposed to agents that promote ER stress, and the ECM generated and assessed for ability to support leukocyte adhesion. HAS = hyaluronan synthase; Vcan = versican; V3 = versican isoform V3; Ox LDL = oxidized low density lipoprotein.
