FIG. 4.

Using MPM and optically cleared tissue, one can obtain a three-dimensional (3D) image of the vessel as a whole (A, B), as well as images of planes perpendicular to the radius; this technique allows effective imaging at depths great enough to traverse the vessel from one wall to the other, a distance of ∼2.5 mm (C–E). Planes within a single wall also reveal the changes in collagen fiber alignment that occur between the outer edge of the vessel and the luminal face, indicating that the ability to image large volumes of tissue does not sacrifice resolution to do so (F–H). Scale bars=1 mm (A–E); 100 μm (F–H).