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Optical sections through a 3D volume (A, B) illustrate the ability to identify regions of interest for sectioning and further analysis noninvasively (C, D). Because the distribution of cells within the reseeded decellularized matrix scaffold is nonuniform, the ability to identify cutting depth a priori avoids loss of potentially important information. Depth into the tissue is 0, 160, and 760 μm, respectively (C–E). Scale bars=500 μm (A, B); 250 μm (C–E).
