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. 2014 Jun 17;14:168. doi: 10.1186/1471-2229-14-168

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Copyright © 2014 Sierocka et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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RDA-cDNA amplicon analysis on 1.5% agarose gels. (A) cDNA of the TESTER (F-female) and the DRIVER (M-male) amplicons are shown. 1 kb marker is on the right of the gel. (B) Difference products (DP) after first (DP^I), second (DP^II), third (DP^III), and fourth (DP^IV) round of subtractive hybridization are presented.100 bp marker is on the left of the gels.