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. 2014 Jun 17;14:168. doi: 10.1186/1471-2229-14-168

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Copyright © 2014 Sierocka et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transcripts levels of PenB_CYSP, PenB_MT2 and PenB_MT3 genes are connected to with archegonia development. Quantitative real-time PCR analyses of three studied genes show different transcripts levels in the vegetative and archegonia bearing parts of female gametophytes grown in natural habitat. All transcript levels were normalized against ACTIN1. As a control of comparable gene expression in the tested female thalli primers amplifying fragment of histone H4 were used. Calculation shows the mean ± SD from three technical replicates.