Table 1.
Effect of cyclopamine on length of regenerating tail
| |
Day 3 |
Day 5 |
||||||
|---|---|---|---|---|---|---|---|---|
| Chemicals | ET | TO | CP | CP + PM | ET | TO | CP | CP + PM |
| Number |
11 |
11 |
12 |
11 |
9 |
9 |
12 |
10 |
| Mean (μm) |
725.3 |
785.2 |
401.2 |
487 |
1528.8 |
1751.6 |
718.4 |
1168.2 |
| SD |
209.3 |
225.7 |
171.9 |
134.1 |
375.2 |
408.1 |
219 |
293.1 |
| P-value (ET) |
- |
0.26 |
0.2 E-4 |
2.4 E-3 |
- |
0.12 |
2.7 E-6 |
1.6 E-2 |
| P-value (TO) |
- |
- |
7.5 E-5 |
6.1 E-4 |
- |
- |
2.2 E-7 |
1.1 E-2 |
| P-value (CP) | - | - | - | 0.1 | - | - | - | 2.7 E-4 |
Tail-amputated st. 48 tadpoles were maintained in water containing ethanol (ET, 0.1%), tomatidine (TO, 2.5 μM) or cyclopamine (CP, 2.5 μM) or cyclopamine plus purmorphamine (PM, 0.25 μM). Mean length of the regenerated tails was determined at day 3 and day 5. Student’s t-test was used to identify differences between indicated groups.