Table 3.
Effect of Cyclopamine on proliferation of regenerating cells
|
Tissues |
Notochord |
Spinal cord |
Mesenchyme |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Chemicals | ET | TO | CP | ET | TO | CP | ET | TO | CP |
| % BrdU+ cells |
52.3 |
54.9 |
59.3 |
61.2 |
57.5 |
35.6 |
66.9 |
70.7 |
36.2 |
| SD |
22.5 |
18.2 |
13.8 |
16.7 |
13.6 |
11.6 |
14.5 |
10.5 |
9.6 |
| number of sections |
18 |
20 |
21 |
18 |
16 |
15 |
14 |
27 |
36 |
| p-value (ET) |
- |
0.65 |
0.19 |
- |
0.24 |
1.0 E-5 |
- |
0.83 |
8.4 E-12 |
| p-value (TO) | - | - | 0.81 | - | - | 2.1 E-5 | - | - | 2.3 E-20 |
Tail-amputated st. 48 tadpoles were maintained in water containing ethanol (ET, 0.1%), tomatidine (TO, 2.5 μM) or cyclopamine (CP, 2.5 μM). Four tadpoles for each condition were fixed at day 2.5 after 12 h BrdU labeling. Mean rate of proliferating cells were determined on sagittal (notochord and spinal cord) or frontal (mesenchymal region) sections. Student’s t-test was used to identify differences between indicated groups.