Table VII.

Percentage of DNA promoter methylation for 6 representative genes in the lungs of 20 Gy thoracic irradiated C3H/HeNHsd mice.

day	TGFb	SOD2	FGF1	VEGFa	CTGF	IGFbp7
0	92.45±1.97	93.45±1.05	95.43±1.51	89.88±0.97	94.93±0.79	95.60±0.56
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p2=0.0127	p2=0.3512	p2=0.1685	p2=0.0002	p2=0.1401	p2=0.4542
2	90.78±1.93	91.28±0.75	94.45±0.44	95.25±0.37	96.75±0.24	94.50±0.42
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p1=0.2695	p1=0.0153	p1=0.2606	p1<0.0001	p1=0.0045	p1=0.0197
	p2=0.0027	p2<0.0001	p2<0.0001	p2=0.0004	p2<0.0001	p2=0.0004
14	95.23±0.86	91.55±0.60	94.40±0.61	95.95±0.26	97.45±0.37	94.00±0.62
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p1=0.0415	p1=0.0201	p1=0.2536	p1<0.0001	p1=0.0012	p1=0.0085
	p2=0.0015	p2=0.3948	p2=0.2093	p2<0.0001	p2=0.3302	p2<0.0001
28	91.35±0.54	95.45±0.54	92.85±0.31	96.35±0.44	97.85±0.34	94.93±0.43
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p1=0.3229	p1=0.0150	p1=0.0391	p1<0.0001	p1=0.0005	p1=0.1037
	p2=0.0251	p2=0.0123	p2=0.3016	p2=0.0006	p2=0.3156	p2=0.0015
75	91.05±0.83	96.43±0.42	94.40±0.43	96.28±0.44	97.63±0.53	94.88±0.33
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p1=0.2383	p1=0.0019	p1=0.2387	p1<0.0001	p1=0.0013	p1=0.0672
	p2=0.0001	p2=0.0022	p2<0.0001	p2<0.0001	p2=0.2840	p2=0.0011
100	87.50±3.11	95.43±0.46	94.20±0.77	96.68±1.00	96.88±0.93	93.35±0.54
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p1=0.0360	p1=0.0138	p1=0.1982	p1=0.0001	p1=0.0188	p1=0.0012
	p2=0.0007	p2=0.0014	p2=0.0021	p2=0.0268	p2=0.0928	p2=0.0336
150	91.10±2.11	97.83±0.91	94.98±1.01	97.20±0.70	96.28±0.81	92.60±0.95
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p1=0.3853	p1=0.0008	p1=0.6372	p1<0.0001	p1=0.0551	p1=0.0016
	p2=0.0020	p2=0.4908	p2=0.2874	p2=0.0063	p2=0.0005	p2=0.0007
200	90.60±1.27	98.33±0.51	94.10±0.65	97.50±0.63	97.33±0.36	91.28±0.53
	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)	(n=4)
	p1=0.1656	p1=0.0002	p1=0.1572	p1<0.0001	p1=0.0015	p1<0.0001
	p2<0.0001	p2=0.7955	p2=0.0056	p2=0.0545	p2=0.0004	p2<0.0001

Data summary using mean ± SD and group comparisons for percent methylation, where p1 is the p-value for the comparison of DNA promoter methylation percentage for the corresponding gene in the lungs of C3H/HeNHsd mice between each day and day 0, p2 is the p-value for the comparison of DNA promoter methylation percentage between C57BL/6NHsd mice and C3H/HeNHsd mice at the corresponding day for the corresponding gene. All p-values were calculated with the two-sided two sample t-test.