Table 1.
Formulation variables and pharmaceutical attributes of PLGA NPs prepared using an emulsion–diffusion–evaporation method.
| Dye | Formula code | NPs formulation variables |
Homogenization speed, rpm | NPs properties, mean ± SD (n ⩾ 5) |
|||||
|---|---|---|---|---|---|---|---|---|---|
| PLGA composition | Dye (% w/w) |
DMAB (% w/v) |
PVA (% w/v) |
Size (nm) | PDI | Zeta potential (mV) | |||
| Rh B | F1 | 50:50 | 20 | 3 | – | 5000 | 422.3 ± 5.52 | 0.37 ± 0.04 | 65.7 ± 1.53 |
| F2 | 50:50 | 20 | 3 | – | 10,000 | 251.5 ± 7.80 | 0.67 ± 0.06 | 67.6 ± 1.19 | |
| F3 | 50:50 | 20 | 3 | – | 15,000 | 155.2 ± 4.73 | 0.48 ± 0.02 | 66.4 ± 1.51 | |
| F4 | 100:0 | 20 | 1 | – | 15,000 | 91.90 ± 2.47 | 0.17 ± 0.01 | 54.7 ± 2.37 | |
| F5 | 75:25 | 20 | 1 | – | 15,000 | 101.1 ± 2.90 | 0.15 ± 0.02 | 50.4 ± 1.52 | |
| F6 | 50:50 | 20 | 1 | – | 15,000 | 105.5 ± 2.92 | 0.15 ± 0.02 | 55.2 ± 2.86 | |
| F7 | 50:50 | 10 | 1 | – | 15,000 | 117.4 ± 0.94 | 0.16 ± 0.01 | 57.0 ± 1.21 | |
| F8 | 50:50 | 5 | 1 | – | 15,000 | 121.7 ± 3.13 | 0.15 ± 0.02 | 54.1 ± 1.44 | |
| FITC | F9 | 50:50 | 5 | 1 | – | 15,000 | 130.9 ± 4.85 | 0.16 ± 0.01 | 66.1 ± 2.28 |
| F10 | 50:50 | 10 | 1 | – | 15,000 | 122.0 ± 3.15 | 0.15 ± 0.02 | 57.1 ± 1.51 | |
| F11 | 50:50 | 20 | 1 | – | 15,000 | 362.7 ± 78.48 | 0.67 ± 0.04 | 36.1 ± 2.09 | |
| F12 | 50:50 | 10 | – | 1 | 15,000 | 367.0 ± 22.46 | 0.41 ± 0.05 | −4.5 ± 0.45 | |