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. Author manuscript; available in PMC: 2014 Jun 30.
Published in final edited form as: J Nucl Med. 2014 Apr 3;55(5):805–811. doi: 10.2967/jnumed.113.134031

FIGURE 1.

FIGURE 1

In vitro characterization of reporter adenovirus. (A) Reporter expression was validated by Western blot in HCT116 cells and by fluorescent cellular immunoassay in PC3-CAR cells 48 h after infection of reporter adenovirus. Supplemental data provide experimental details. (B–D) Functionality of each reporter was verified in HCT116 and PC3-CAR cells by 3H-ganciclovir (HGCV) uptake (B), 125I uptake (C), and YC-I-27 binding (D). Data were normalized to cellular GFP expression and plotted as mean ± SE of mean of 3 replicates. *P < 0.01.