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. 2014 Jun 20;6(6):2428–2443. doi: 10.3390/v6062428

Figure 5.

Figure 5

The efficiency of E gene insertion in the pBAC-C-KCE base using MAGIC and characterization of the recombinant C-KCE-E viruses. (A) Detection of the E gene insertion in pBAC-C-KCE by PCR. The marker used was DL2000. (B) Detection of the E gene insertion in the recombinant viruses by PCR. The numbers show the passages of C-KCE-E. The marker used was DL5000. (C) Confirmation of the expression of the E protein in C-KCE-E-infected chicken embryo fibroblasts (CEFs) by using immunofluorescence. (D) Detection of the expression of E protein in C-KCE-E-infected CEFs by western blotting.