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. 2014 Jun 30;5:314. doi: 10.3389/fmicb.2014.00314

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Copyright © 2014 Chiu, Healer, Thompson, Chen, Kaul, Savergave, Raghuvanshi, Li Wai Suen, Siba, Schofield, Mueller, Cowman and Hansen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunization of rabbits with recombinant PfRh5 and PfRip5 induces strain-transcending parasite growth inhibitory antibodies. The PfRh5 ectodomain sequence and the C-terminal PfRipr fragment were cloned and expressed in E. coli and baculovirus-infected Hi-5 cells, respectively. SDS-PAGE analysis (A) of the purified recombinant proteins. Total IgG purified from sera of PfRh5 (B) and PfRipr (C) immunized rabbits was added to P. falciparum 3D7, W2Mef, and FCR3 cultures to determine their capacity to inhibit parasite growth.